Supplemental Movie EV5. Immune cells display rear Myosin II activity that may aid nucleokinesis during migration in vivo. Rear-localised Myosin II (cyan) during hemocyte (nucleus, yellow) rear retraction and nucleokinesis in a 75h APF vessel. The images were acquired every 22 seconds; scalebar is 10 m. Genotype was SqhAX3; Srp>nRFP; sqh-Sqh-GFP.